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4 edition of Role of renin substrate in apparent prorenin convertase effects observed in rat plasma found in the catalog.

Role of renin substrate in apparent prorenin convertase effects observed in rat plasma

William Neeson

Role of renin substrate in apparent prorenin convertase effects observed in rat plasma

by William Neeson

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Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.)--University of Toronto, 1990.

SeriesCanadian theses = Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches.
ID Numbers
Open LibraryOL14890987M
ISBN 100315585072
OCLC/WorldCa31289985

Abstract. Most proteolytic enzymes in plasma circulate as inactive precursors. These proenzymes are activated by limited proteolysis during physiological processes such as coagulation, fibrinolysis and complement-mediated reactions [1, 2], For a long time most physiologists and clinicians were interested in renin as a classical circulating hormone [3], despite the well-known fact that renin is Author: F. H. M. Derkx, B. N. Bouma, H. L. Tan-Tjiong, A. J. Man in’t Veld, J. H. B. de Bruyn, G. J. Wenting. Role of C. elegans TAT-1 Protein in Maintaining Plasma Membrane Phosphatidylserine Asymmetry Monica Darland-Ransom, 1Xiaochen Wang, * Chun-Ling Sun,1* James Mapes, Keiko Gengyo-Ando, 2Shohei Mitani, Ding Xue1† The asymmetrical distribution of phospholipids on the plasma membrane is critical for maintaining cell.

A. the relative affinities of substrate for the T and R conformations plays an important role in the cooperativity of the reaction. B. the equilibrium between the T and R conformations plays a minor role. C. the enzymatic activity of the T conformation is considerably higher than that of the R form. Department of Biology () T.H. Morgan Building Lexington KY

the plasma urate concentrations. Urateconcentrations returned to pre-drug values by day 12 following the administration of all the drugs except for phenylbutazone (Fig. 1). Nosignificant depression of plasma urate was observed after the administra-tion of colchicine or indomethacin. TABLE I.-Effect of Drugs on Plasma Usate No Of Cited by:   The renin-angiotensin system (RAS) has become increasingly complex. New components have been identified, and additional roles for angiotensin peptides and their receptors are being uncovered. A functional (pro)renin receptor has been cloned that acts as (pro)renin cofactor on cell surface, enhancing the efficiency of angiotensinogen cleavage by (pro)renin and unmasking prorenin Cited by:


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Role of renin substrate in apparent prorenin convertase effects observed in rat plasma by William Neeson Download PDF EPUB FB2

Renin substrate (angiotensinogen) preparations in the determination of prorenin and renin: Evidence for extrarenal plasma prorenin and its renal 'convertase'. Renin is an aspartyl protease synthesized as prorenin, a proenzyme that contains an additional 43–amino acid N-terminal fragment.

The physiological maturation of prorenin into active renin takes place exclusively in the juxtaglomerular cells of the kidney. Renin has high substrate specificity, and its only known substrate is by: Renin substrate (angiotensinogen) preparations in the deteminat~on of prorenin and renin: evidence for extrarenal plasma prorenin and its renal "convertase".

Can. The measurement of renin activity, concentration, and substrate in rat plasma has been performed by radioimmunoassay of the angiotensin I generated during incubation of plasma in vitro.

The sensitivity of the method permits measurements of all three renin parameters in small plasma samples, allowing sequential determinations to be performed in Cited by: The fact that PreR-Co does not process plasma prorenin is in accordance with previous reports suggesting that plasma prorenin is not the source of circulating active renin (Mullins et al.,Lenz et al., ).

The role of plasma prorenin and extrarenal tissues prorenin is obscure and further investigations are necessary to clarify this by: 3. The role of renin substrate in hypertension.

Gordon DB. A positive correlation between blood pressure and renin substrate concentration is found in a wide variety of conditions in human beings and in animal experiments.

The evidence concerning this relationship is reviewed and the exceptions are by: Species Differences in the Kinetics of the Renin-Substrate Reaction in Plasma KENDALL B.

WALLACE, JEFFREY L. OSBORN, AND MICHAEL D. BAILIE In view of the substrate-dependence of renin, it was of interest to examine the kinetics of the renin-angiotensinogen reaction in plasma of various species to establish differences in : Kendall B.

Wallace, Jeffrey L. Osborn, Michael D. Bailie. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a aa proform (aa 22 - ).

In the kidney, prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-renin shares 70% and 85% aa sequence identity with human and rat pro-renin, respectively.5/5(1). Prorenin and renin are both involved in atherosclerosis. However, the role of plasma prorenin and renin in the development and progression of coronary artery disease (CAD) is still not clear.

Thus, we aimed to examine the relationships among plasma prorenin concentration, CAD and clinical parameters.

We measured plasma prorenin and renin concentrations and other parameters in 85. Renin substrate protein is a member of the serpin family.

Discovery The history of the discovery of the renin-angiotensin system began in with the studies made by Tigerstedt and Bergman, who reported the pressor effect of renal extracts; they named the renal substance renin based on its origin.

Background: Prorenin and renin are both involved in atherosclerosis. However, the role of plasma prorenin and renin in the development and progression of coronary artery disease (CAD) is still not clear.

Thus, we aimed to examine the relationships among plasma prorenin concentration, CAD and clinical parameters. Methods: We measured plasma Cited by: 3.

Renin-angiotensin system and cancer. Research into the role of the RAS in cancer has gained momentum in recent times.

This is underscored by the observation of dysregulation of the RAS in different types of cancers, and the observed efficacy of RAS modulators on cancer in both cancer models and cancer patients []. Pre-treatment Km values varied greatly among the normal subjects. The mean Km was μg/ml.

Under these conditions, the in vitro reaction of renin with physiologic concentrations of renin substrate will proceed at only 70% of its maximal velocity.

Following estrogen administration, Km doubled to a value of μg/ml. Vmax increased by 81%.Cited by: plasma renin activity: Definition Renin is an enzyme released by the kidney to help control the body's sodium-potassium balance, fluid volume, and blood pressure.

Purpose Plasma renin activity (PRA), also called plasma renin assay, may be used to screen for high blood pressure (hypertension) of kidney origin, and may help plan treatment of. buffer and endogenous renin in human plasma. The most striking observation made was the detection of a plasma inhibitor to the renin-antirenin reaction.

This inhibitor later proved to have the same molecular size as renin substrate, but could be separated from the latter by ion-exchange chromatography.

When. plasma renin activity, the plasma renin substrate, as well as the renal renin content were determined. The kidneys of the other animals were processed for elec-tron microscopic examination of the structure of the juxtaglomerular cells.

Arterial blood was drawn by carotid cannulation from each rat at the end of the experimental period. The classical renin angiotensin system with the generation of different peptides and their physiological effects has been presented in Figure 1.

The systemic or classical renin angiotensin system has usually been viewed as the blood-borne cascade whose ultimate product Ang-II pl ays the pivotal endocrine role.

Thanks for your posts. I have read that HTN associated with increased renin responds favorably with ACE inhibitor therapy. So I guess I can then see why ACE inhibitors would want to increase renin levels. I'll try to leave it at that.

Just got so confused because renin is what starts the whole RAAS process to increase bp. are: 1) the systemically circulating enzyme renin and its precursor, prorenin, derived from the juxtaglomerular cells of the renal afferent arterioles; 2) angiotensinogen (AGT), a high molecular weight protein, released from the liver, which is the only substrate of renin and.

Renin secretion is stimulated by a decrease in arterial blood pressure, a decrease in sodium chloride levels in kidney nephrons, or sympathetic nervous system activity.

The substrate of renin, angiotensinogen, is a amino acid residue in humans. renin release was calculated from the increase in renin activity in the supernatants using a linear regression program.

Renin Activity Assay Aliquots of cell extracts and cell-conditioned buffer were incubated for h at 37°C using plasma of bilat- erally nephrectomized male rats as renin substrate.

The.As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources.

Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized by:   Moreover, purified renin substrate, like erythropoietin, causes the dose-dependent increase of haemoglobin F in cultured human erythroid leukaemia K .